K m (MT) and the k cat were obtained by curve fitting with DeltaGraph with the following r values: GST-NOD320, 0.97 NOD DTW, 0.92 and NOD “DR2”, 0.96. Wild-type NOD-GST, NOD DTW, and NOD “DR2” are represented by filled circles, diamonds, and squares, respectively. (C) ATPase rates of various 50 nM GST-NOD fusions were measured and calculated as in B.
The solid line was obtained from the curve-fitting procedure (r = 0.97). The microtubule dimer concentration that led to half-maximum ATPase activation K m (MT) and the k cat were obtained by curve fitting with DeltaGraph. (B) ATPase rates of 50 nM GST-NOD320 were determined in the presence of various levels of polymerized tubulin and 0.5 mM ATP. The inset shows an SDS-PAGE gel showing molecular weight standards and the first two elutions of GST-NOD320. The microtubule dimer concentration that led to half-maximum ATPase activation Km (MT) and the kcat were obtained by curve fitting with DeltaGraph. The data was fit to the Michaelis-Menten kinetic model and the solid line was obtained from this curve-fitting procedure. These values were plotted by DeltaGraph and K m values were obtained by curve fitting. We used a recently described sigmoidal equation to curve-fit the P-V data sets and objectively define the point. (A) ATPase rate of 50 nM GST-NOD320 was measured in the presence of subsaturating levels of microtubules to determine the apparent K m for ATP. * Please call Manufacturer for compatibility.Determination of the K m (ATP), K m (MT) for the minimal catalytic core of NOD (GST-NOD320): biochemical characterization of two NOD mutant proteins.
Deltagraph curvefit mac os x#
* Share your results with the highest quality output, both onscreen and in print, thanks to native Mac OS X rendering and PANTONE™ color matching. DeltaGraph 6 offers scientific and business charts, analysis tools. If this is the case - you will find information about these extensions in the list. In addition, you will need the following scripts: hrf. It assumes you have some basic knowledge of Matlab and/or DeltaGraph and that you have those tools available to you. DeltaGraph's Chart Advisor shows you the charting options for your data, and will even recommend a chart type and style based on the type of data and audience. Program DeltaGraph may also be used to convert files between different formats. This tutorial will describe fitting an hrf to your data, using two different tools: Matlab's Curve Fitting Toolbox and DeltaGraph. Nearly every chart element is customizable in color, font, style, and location. With over 80 different chart types and 200 different styles, both 2-D and 3-D, you have a choice of business and technical charts. * Visualize your results the way you want. * Analyze your data with our Formula Builder and 50 mathematical and statistical functions or, fit a curve to your data with advanced regression tools.
Deltagraph curvefit for mac os#
Analyze, visualize and customize your numbers, efficiently and with high quality out put using DeltaGraph 5 for Mac OS 9 and OS X.
DeltaGraph's 15 year presence in the marketplace is testimony to its power and flexibility. DeltaGraph 6.0.19 and earlier crash when printing or exporting graphics, when run in Yosemite 10.10.0. Please take a minute to read the provided Instructions before you proceed.
Deltagraph curvefit install#
Red Rock Software is proud to bring you DeltaGraph 5, the most comprehensive charting application for Mac OS 9 and OS X. All DeltaGraph for Mac 6 users may download and install this FREE update. Specialists in Graphics Software and Hardware
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